This site is a development preview. As such the content and styling may not be final and is subject to change before going into production. To see more information about the redesign click here.

openPrimeR

This is the development version of openPrimeR; for the stable release version, see openPrimeR.

Multiplex PCR Primer Design and Analysis

Bioconductor version: Development (3.18)

An implementation of methods for designing, evaluating, and comparing primer sets for multiplex PCR. Primers are designed by solving a set cover problem such that the number of covered template sequences is maximized with the smallest possible set of primers. To guarantee that high-quality primers are generated, only primers fulfilling constraints on their physicochemical properties are selected. A Shiny app providing a user interface for the functionalities of this package is provided by the 'openPrimeRui' package.

Author: Matthias Döring [aut, cre], Nico Pfeifer [aut]

Maintainer: Matthias Döring <matthias-doering at gmx.de>

Citation (from within R, enter citation("openPrimeR")):

Installation

To install this package, start R (version "4.3") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

# The following initializes usage of Bioc devel
BiocManager::install(version='devel')

BiocManager::install("openPrimeR")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("openPrimeR")
openPrimeR HTML R Script
Reference Manual PDF
NEWS Text

Details

biocViews Coverage, MultipleComparison, Software, Technology
Version 1.23.0
In Bioconductor since BioC 3.6 (R-3.4) (6 years)
License GPL-2
Depends R (>= 4.0.0)
Imports Biostrings(>= 2.38.4), XML (>= 3.98-1.4), scales (>= 0.4.0), reshape2 (>= 1.4.1), seqinr (>= 3.3-3), IRanges(>= 2.4.8), GenomicRanges(>= 1.22.4), ggplot2 (>= 2.1.0), plyr (>= 1.8.4), dplyr (>= 0.5.0), stringdist (>= 0.9.4.1), stringr (>= 1.0.0), RColorBrewer (>= 1.1-2), DECIPHER(>= 1.16.1), lpSolveAPI (>= 5.5.2.0-17), digest (>= 0.6.9), Hmisc (>= 3.17-4), ape (>= 3.5), BiocGenerics(>= 0.16.1), S4Vectors(>= 0.8.11), foreach (>= 1.4.3), magrittr (>= 1.5), distr (>= 2.6), distrEx (>= 2.6), fitdistrplus (>= 1.0-7), uniqtag (>= 1.0), openxlsx (>= 4.0.17), grid (>= 3.1.0), grDevices (>= 3.1.0), stats (>= 3.1.0), utils (>= 3.1.0), methods (>= 3.1.0)
Linking To
Suggests testthat (>= 1.0.2), knitr (>= 1.13), rmarkdown (>= 1.0), devtools (>= 1.12.0), doParallel (>= 1.0.10), pander (>= 0.6.0), learnr (>= 0.9)
System Requirements MAFFT (>= 7.305), OligoArrayAux (>= 3.8), ViennaRNA (>= 2.4.1), MELTING (>= 5.1.1), Pandoc (>= 1.12.3)
Enhances
URL
See More
Depends On Me openPrimeRui
Imports Me
Suggests Me
Links To Me
Build Report  

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package openPrimeR_1.23.0.tar.gz
Windows Binary openPrimeR_1.23.0.zip
macOS Binary (x86_64) openPrimeR_1.23.0.tgz
macOS Binary (arm64) openPrimeR_1.23.0.tgz
Source Repository git clone https://git.bioconductor.org/packages/openPrimeR
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/openPrimeR
Bioc Package Browser https://code.bioconductor.org/browse/openPrimeR/
Package Short Url https://bioconductor.org/packages/openPrimeR/
Package Downloads Report Download Stats