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spiky

This is the development version of spiky; for the stable release version, see spiky.

Spike-in calibration for cell-free MeDIP

Bioconductor version: Development (3.18)

spiky implements methods and model generation for cfMeDIP (cell-free methylated DNA immunoprecipitation) with spike-in controls. CfMeDIP is an enrichment protocol which avoids destructive conversion of scarce template, making it ideal as a "liquid biopsy," but creating certain challenges in comparing results across specimens, subjects, and experiments. The use of synthetic spike-in standard oligos allows diagnostics performed with cfMeDIP to quantitatively compare samples across subjects, experiments, and time points in both relative and absolute terms.

Author: Samantha Wilson [aut], Lauren Harmon [aut], Tim Triche [aut, cre]

Maintainer: Tim Triche <trichelab at gmail.com>

Citation (from within R, enter citation("spiky")):

Installation

To install this package, start R (version "4.3") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

# The following initializes usage of Bioc devel
BiocManager::install(version='devel')

BiocManager::install("spiky")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("spiky")
Spiky: Analysing cfMeDIP-seq data with spike-in controls HTML R Script
Reference Manual PDF
NEWS Text

Details

biocViews DNAMethylation, DifferentialMethylation, Normalization, Preprocessing, QualityControl, Sequencing, Software
Version 1.7.0
In Bioconductor since BioC 3.14 (R-4.1) (2 years)
License GPL-2
Depends Rsamtools, GenomicRanges, R (>= 3.6.0)
Imports stats, scales, bamlss, methods, tools, IRanges, Biostrings, GenomicAlignments, BlandAltmanLeh, GenomeInfoDb, BSgenome, S4Vectors, graphics, ggplot2, utils
Linking To
Suggests covr, testthat, rmarkdown, markdown, knitr, devtools, BSgenome.Mmusculus.UCSC.mm10.masked, BSgenome.Hsapiens.UCSC.hg38.masked, BiocManager
System Requirements
Enhances
URL https://github.com/trichelab/spiky
Bug Reports https://github.com/trichelab/spiky/issues
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Build Report  

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package spiky_1.7.0.tar.gz
Windows Binary spiky_1.7.0.zip
macOS Binary (x86_64) spiky_1.7.0.tgz
macOS Binary (arm64) spiky_1.7.0.tgz
Source Repository git clone https://git.bioconductor.org/packages/spiky
Source Repository (Developer Access) git clone git@git.bioconductor.org:packages/spiky
Bioc Package Browser https://code.bioconductor.org/browse/spiky/
Package Short Url https://bioconductor.org/packages/spiky/
Package Downloads Report Download Stats