gCrisprTools

Suite of Functions for Pooled Crispr Screen QC and Analysis

Bioconductor version: Release (3.17)

Set of tools for evaluating pooled high-throughput screening experiments, typically employing CRISPR/Cas9 or shRNA expression cassettes. Contains methods for interrogating library and cassette behavior within an experiment, identifying differentially abundant cassettes, aggregating signals to identify candidate targets for empirical validation, hypothesis testing, and comprehensive reporting. Version 2.0 extends these applications to include a variety of tools for contextualizing and integrating signals across many experiments, incorporates extended signal enrichment methodologies via the "sparrow" package, and streamlines many formal requirements to aid in interpretablity.

Author: Russell Bainer, Dariusz Ratman, Steve Lianoglou, Peter Haverty

Maintainer: Russell Bainer <russ.bainer at gmail.com>

Citation (from within R, enter citation("gCrisprTools")):

Installation

To install this package, start R (version "4.3") and enter:

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("gCrisprTools")

For older versions of R, please refer to the appropriate Bioconductor release.

Documentation

To view documentation for the version of this package installed in your system, start R and enter:

browseVignettes("gCrisprTools")

Contrast_Comparisons_gCrisprTools	HTML	R Script
Example_Workflow_gCrisprTools	HTML	R Script
gCrisprTools_Vignette	HTML	R Script
Reference Manual	PDF
NEWS	Text

Details

biocViews	BiomedicalInformatics, CRISPR, CellBiology, DifferentialExpression, ExperimentalDesign, FunctionalGenomics, GeneSetEnrichment, Genetics, ImmunoOncology, MultipleComparison, Normalization, Pharmacogenetics, Pharmacogenomics, PooledScreens, Preprocessing, QualityControl, RNASeq, Regression, Software, SystemsBiology, Visualization
Version	2.6.0
In Bioconductor since	BioC 3.4 (R-3.3) (7 years)
License	Artistic-2.0
Depends	R (>= 4.1)
Imports	Biobase, limma, RobustRankAggreg, ggplot2, SummarizedExperiment, grid, rmarkdown, grDevices, graphics, methods, ComplexHeatmap, stats, utils, parallel
Linking To
Suggests	edgeR, knitr, AnnotationDbi, org.Mm.eg.db, org.Hs.eg.db, BiocGenerics, markdown, RUnit, sparrow, msigdbr, fgsea
System Requirements
Enhances
URL

Depends On Me
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Build Report

Package Archives

Follow Installation instructions to use this package in your R session.

Source Package	gCrisprTools_2.6.0.tar.gz
Windows Binary	gCrisprTools_2.6.0.zip
macOS Binary (x86_64)	gCrisprTools_2.6.0.tgz
macOS Binary (arm64)	gCrisprTools_2.6.0.tgz
Source Repository	git clone https://git.bioconductor.org/packages/gCrisprTools
Source Repository (Developer Access)	git clone git@git.bioconductor.org:packages/gCrisprTools
Bioc Package Browser	https://code.bioconductor.org/browse/gCrisprTools/
Package Short Url	https://bioconductor.org/packages/gCrisprTools/
Package Downloads Report	Download Stats